TABLE 1.

Enzyme activities and ethanol production in transformed Synechococcus sp. strain PCC 7942^a

Cell line	Activity (nmol · min−1 · mg of total protein−1)		Ethanol concn (μM)
	PDC	ADH
pCB4 (control)	NDb	ND	ND
pCB4-Rpa	130	168	1,370
pCB4-LRpa	136	140	1,540
pCB4-LR(TF)pa	234	168	1,710

^aThe shuttle vector pCB4 (control) and the constructs containing the pdc and adh genes were used individually to transform cyanobacterial cells. Transformed cells were grown in 50 ml of culture medium at 30°C in the presence of light for 21 days (final OD₇₃₀, approximately 1.5). Cells were harvested to assay in order to determine the PDC and ADH activities in the cell lysates. The ethanol concentration in the culture medium was determined. The values are means of two or three values obtained with different samples from replicate cultures. For each replicate culture the values differed by less than 5%. 

^bND, not detected.