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. 2022 Apr 26;11(9):1464. doi: 10.3390/cells11091464

Table 4.

Effects of MSC coculture with neural cells in vitro.

Neural Cells Source MSCs Source Additional Information Observed Result Bibliography
NSCs and NPCs
Adult brain (m) AT (r) Coculture as spheres, on chitosan surface
  • -

    Promotion of NSC survival in vitro and in vivo

 [148]
Adult hippocamp, ventrical zones (r) BM (r) Adherent culture of BM-MSCs and NSCs
  • -

    Increased oligodendral differentiation of NSCs

 [150]
Adult hippocamp (r) BM (h) NSCs cultured over MSCs
  • -

    Stimulation of NSC differentiation into astrocytes and oligodendrocytes

 [149]
Brain (m) AT (m) NSCs were irradiated before,
and the transwell system coculture was used with MSCs
  • -

    Higher survival of irradiated NSCs after coculture

  • -

    Higher clonogenicity of irradiated NSCs after coculture

 [151]
Hippocampal NPCs (r) MSCs, ns (h) Ns
  • -

    NPCs treated with amyloid-β (Alzheimer disease model)

  • -

    Increased neurogenesis of treated NPCs and enhanced neuronal differentiation

 [152]
Whole brain extracts (r) BM (r) Lack of mitogens in medium
  • -

    Preservation of NSCs stemness

 [147]
Fetal tissue (h) BM (h) NSCs over MSCs or MSCs over NSCs,
With or without Notch-1
  • -

    Increased expression of Notch-1 and Hes-1 by NSCs

  • -

    Increased proliferation of NSCs

  • -

    Enhanced stemness of NSCs

 [145]
Fetal tissue (h) BM (h) Transwell system coculture
  • -

    Promotion of neuronal differentiation of BM-MSCs

  • -

    Increased NGF and BDNF secretion

 [144]
Cell line (ATCC, Catalog #CRL-2925), (m) AT (h) Mixed coculture,
in vitro ischemia model
  • -

    Inhibition of NSCs apoptosis

 [146]
NPC cell line (Millipore) (r) WJ termed and pretermed (h) Direct coculture and transwell coculture
  • -

    Increased expression of glial markers of NPCs in direct coculture

 [10]
NSCs derived from iPSCs (h) AT (h) Culture inserts, MSCs over NSCs
  • -

    Prevention of lipopolysaccharide-induced activation of nuclear factor-κB (NF-κB) in NSCs

  • -

    Smaller scars and better preservation of β-III tubulin-positive axons after transplantation of NSCs and MSCs to rats with spinal cord injuries

 [153]
Differentiated cells
Fetal brain astrocytes (h) BM (h) MSC neurosphere
  • -

    Synapse formation

  • -

    Generation of electrically active neurons

 [106]
Astrocytes (m) AT (h) Astrocytes derived from the ALS mice model,
Transwell system coculture
  • -

    Enhanced glutamate uptake

  • -

    Increased secretion of neuroprotective agents

 [154]
Fetal hippocamp, neurons (m) UCB (h) Transwell system coculture, neurons treated with Aβ42 (the Alzheimer disease model)
  • -

    Prevention of reduction in synaptic density caused by Aβ42 peptide in the Alzheimer disease model

 [155]
Neurons differentiated from SH-SY5Y (h) UCB (h) OGD-stressed neurons, MSCs inserts
  • -

    Rescue of neuronal cells from apoptosis

 [156]
Organotypic hippocampal slices (r) WJ (h) OGD-stressed hippocampal slices
  • -

    Neuroprotective effect of MSCs

  • -

    Enhanced neural differentiation of WJ and WJ-MSCs

 [75]
Organotypic hippocampal slices (r) WJ (h) OGD-stressed hippocampal slices, transwell system coculture
  • -

    Decreased apoptosis and vascular atrophy of hippocamp

 [6]

Abbreviations: h—human, m—mouse, r—rat; AT—adipose tissue, BDNF—brain-derived neurotrophic factor, BM—bone marrow, iPSCs—induced pluripotent stem cells, MSCs—mesenchymal stem cells, NGF—nerve growth factor, NPCs—neural progenitor cells, NSCs—neural stem cells, OGD—oxygen glucose deprivation, UCB—umbilical cord blood, WJ—Wharton jelly.