Figure 4.
Normalized Intrinsic fluorescence. (a) HβA1 in the absence of metal ions (black) and in the presence of Zn(II) (red). (b) HβA1 in the absence of metal ions (black) and in the presence of Cu(II) (blue). (c) HβB2 in the absence of metal ions (black) and in the presence of Zn(II) (red). (d) HβB2 in the absence of metal ions (black) and in the presence of Cu(II) (blue). The emission spectrum was recorded in the range of 300 and 500 nm using an excitation wavelength of 295 nm at 37 °C. (Replica spectra are shown in Figure S7).