Figure 8.

RNA-seq identifies PGR-regulation of stromal tissue. RNA-seq was performed on ovarian stromal tissue at 8 h post-hCG. (A) Volcano plot demonstrating the 104 differentially expressed genes (logFC ≥ 0.5 and padj ≤ 0.05) between PRKO and WT ovarian stromal tissue. Blue depicts downregulated and red depicts upregulated genes in PRKO stroma. (B) Example of Plin4, Pla2g5 and Edn2 differential gene expression as visualized by number of reads mapped on the gene. (C) “Function and diseases” dysregulated in PRKO ovarian stroma based on IPA analysis of stromal DEGs. Similar functions are grouped and shown as log p-value (size), the number of DEGs associated with the function (color) and z-score indicating degree of downregulation (X-axis).