Table 2.

Mean percentage egg weight loss (PEWL) in the 0 to 12, 12 to 18, and 0 to 18 days of incubation (doi) intervals, hatchability of injected eggs containing viable embryos (HI), and mean straight run hatchling BW at 22 doi in ts-11MG exposed diluent-injected control (EC) eggs and those injected with 3.63 × 10¹, 3.63 × 10², 3.63 × 10³, and 3.63 × 10⁴ cfu of the ts-11MG vaccine (ts-11MGV).

Treatment	PEWL0 to 12 doi 6	PEWL12 to 18 doi	PEWL 0 to 18 doi	HI 7	Hatchling BW 8
	(%)				(g)
EC 1	7.09	3.94	11.08	97.24	39.81
3.63 × 101 cfu 2	7.09	3.59	10.67	97.48	39.70
3.63 × 102 cfu 3	7.15	4.00	11.12	96.15	40.00
3.63 × 103 cfu 4	7.13	4.15	11.29	96.43	39.94
3.63 × 104 cfu 5	7.06	4.01	11.08	97.93	40.16
Source of Variation
Pooled SEM	0.090	0.196	0.207	1.463	0.299
p-value	0.856	0.080	0.063	0.727	0.595

¹ At 18 doi, embryonated eggs injected with Marek’s diluent were incubated in the same hatcher with ts-11MGV injected eggs. ² 10¹ cfu (colony forming unit) of ts-11MG was a 50 μL volume of a 10⁻⁴ dilution from a resuspended vial of vaccine. ³ 10² cfu of ts-11MGV was a 50 μL volume of a 10⁻³ dilution from a resuspended vial of vaccine. ⁴ 10³ cfu of ts-11MGV was a 50 μL volume of a 10⁻² dilution from a resuspended vial of vaccine. ⁵ 10⁴ cfu of ts-11MGV was a 50 μL volume of a 10⁻¹ dilution from a resuspended vial of vaccine. ⁶ PEWL was calculated based on the difference between the initial and final weight of 30 eggs in each of 12 replicate flats in each (0 to 12), (12 to 18), and (0 to 18) doi period. ⁷ Average percentage of hatchability of viable injected eggs containing embryos that hatched successfully and were alive at pull time. ⁸ Hatchling BW was calculated from approximately 20 chicks in each of 12 replicate basket sections in each treatment.