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. 2022 Apr 24;11(9):1442. doi: 10.3390/cells11091442

Figure 3.

Figure 3

RT-PCR analysis of indicated transcripts in Daoy cells transduced with lentiviral vectors expressing MeCP2 isoforms. CT values for (A) MECP2E1, (B) MECP2E2, (C) BDNF, (D) 45S-pre-rRNA, (E) 28S rRNA, (F) 18S rRNA were normalized to the housekeeping gene GAPDH. Fold changes were determined relative to average non-transduced Daoy cell controls. Fold change values were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. n = 5, and data are reported as mean ± SEM; *: p < 0.05, **: p < 0.01, and ****: p < 0.0001 (* indicates the difference from control; # indicates the difference from EF1α-EGFP; δ indicates the difference from EF1α-E1; γ indicates the difference from EF1α-E2; $ indicates the difference from EF1α-E1 + E2, respectively). The p = 0.13 value in (G) is higher than the statistical significance of * p < 0.05. For (G,H), normalization has been carried out with U6 microRNA.