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. 2022 Apr 24;11(9):1442. doi: 10.3390/cells11091442

Figure 4.

Figure 4

Expression of nascent and steady-state MECP2E1 and MECP2E2 transcripts in Daoy cells transduced with lentiviral vectors expressing MeCP2 isoforms. Nascent RNA was prepared from cells labelled with EU for 1 h, 6 h, and 24 h. Total RNA was prepared from unlabeled cells collected at the same time points. Gene expression was analyzed by RT-PCR for (A) MECP2E1 transcripts and (B) MECP2E2 transcripts. CT values were normalized to the housekeeping gene GAPDH, and fold change was determined relative to average non-transduced control cells with n = 3, and data are reported as mean ± SEM. Fold change values were analyzed by two-way ANOVA followed by Tukey’s multiple comparisons test. The levels of significance are *: p < 0.05, **: p < 0.01, ***: p < 0.001, and ****: p < 0.0001.