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. 2022 Apr 24;11(9):1442. doi: 10.3390/cells11091442

Figure 5.

Figure 5

MTT cell survival assay and Western blot analysis of the E1 + E2-transduced cells treated with MG132. (A) Cell survival MTT assays were performed at 3 time points of 6 h, 12 h, and 18 h to determine the cell death/cell survivability with various incubation times and MG132 concentrations. All conditions were performed in n = 5, and data are reported as mean ± SEM with two-way ANOVA analysis, followed by Dunnett’s multiple comparisons test with significances of ****: p < 0.0001. (B) Western blot analysis of the E1 + E2-transduced Daoy cells treated with 2.5 µM of MG132, using anti-MeCP2E1 and anti-MeCP2E2 (h: hour). GAPDH loading control is shown as a housekeeping protein.