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. 2022 Apr 24;11(9):1442. doi: 10.3390/cells11091442

Figure 8.

Figure 8

Expression of the nascent and steady-state 45S pre-rRNA and 28S and 18S rRNA transcripts in Daoy cells transduced with lentiviral vectors expressing MeCP2 isoforms. Nascent RNA was prepared from cells labelled with EU for 1 h, 6 h, and 24 h. Total RNA was prepared from unlabeled cells collected at the same time points. Gene expression was analyzed by RT-PCR for (A) 45S pre-rRNA transcripts, (B) 28S rRNA transcripts, and (C) 18S rRNA transcripts. CT values were normalized to the housekeeping gene GAPDH, and fold change was determined relative to average non-transduced control cells. n = 3, and data are reported as mean ± SEM. Fold change values were analyzed by two-way ANOVA followed by Tukey’s multiple comparisons test: *: p < 0.05 and **: p < 0.01 (* indicates the difference from control; # indicates the difference from EF1α-EGFP, respectively).