Table 1.
Primary antibodies used in Western blot and immunofluorescence.
| Primary Antibody | Application and Dilution | Description | Source |
|---|---|---|---|
| c-Myc | WB 1:1000 | mouse monoclonal | Thermo Fisher Scientific, A21280 |
| c-Myc | IF 1:50 | mouse monoclonal | Thermo Fisher Scientific, MA5-12080 |
| MeCP2E1 | WB, IF 2 μg/mL | chicken polyclonal | Custom-made (Rastegar lab) [16,17] |
| MeCP2E2 | WB, IF 2 μg/mL | chicken polyclonal | Custom-made (Rastegar lab) [17] |
| MeCP2 (C-terminal) | WB 1:1000, IF 1:200 | rabbit polyclonal | Thermo Fisher Scientific, PA5-12234 |
| GAPDH | WB 1:7500 | mouse monoclonal | Santa Cruz Biotechnology, SC-47724 |
| eIF4E | WB 1:2000 | rabbit monoclonal | Sigma, E5906 |
| p-eIF4E | WB 1:1000 | rabbit monoclonal | Sigma, SAB4504389 |
| 4E-BP1 | WB 1:1000 | rabbit monoclonal | Cell Signaling Technology, 9644 |
| p-4EBP1 | WB 1:1000 | rabbit monoclonal | Cell Signaling Technology, 2855 and 9451 |
| MeCP2 | WB 1:1000 | rabbit monoclonal | Cell Signaling Technology, 3456 |
| AKT | WB 1:1000 | rabbit monoclonal | Cell Signaling Technology, 9272S |
| p-AKT | WB 1:500 | rabbit monoclonal | Cell Signaling Technology, 2965S |