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. 2022 Apr 24;11(9):1442. doi: 10.3390/cells11091442

Table 2.

List of secondary antibodies used in Western blot and immunofluorescence.

Secondary Antibody Application and Dilution Source
HRP-conjugated anti-rabbit IgG WB 1:5000 Sigma A6154
Peroxidase-AffiniPure sheep anti-mouse IgG WB 1:7500 Jackson ImmunoResearch 115-035-174
Peroxidase-AffiniPure goat anti-chicken IgY WB 1:7500 Jackson ImmunoResearch 103-035-155
Alexa Fluor 594 conjugated goat anti-chicken IgY IF 1:1000 Invitrogen, Thermo Fisher Scientific, A11042
Alexa Fluor 594 conjugated goat anti-rabbit IgG IF 1:1000 Invitrogen, Thermo Fisher Scientific, A11037
Alexa Fluor 488 conjugated goat anti-mouse IgG IF 1:1000 Invitrogen, Thermo Fisher Scientific, A11017
Anti-Rabbit IgG Peroxidase antibody produced in goat WB 1:5000 Sigma, A6154
Peroxidase AffiniPure Goat Anti-Mouse IgG WB 1:7000 Jackson ImmunoResearch Laboratories, 115-035-174