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. 2022 May 6;23(9):5216. doi: 10.3390/ijms23095216

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CLSM images of conjugated enzymes (a) CBHII, (b) Cel45A, (c) LPMO9D, (d) CDH, (e) GLOX on delignified poplar cross-sections showing fiber cells. Enzyme fluorescence is colored in magenta. Lignin autofluorescence is shown in green. The merged column displays the overlay of enzyme and autofluorescence channels. Graphs show the overlay profile (dash line) of line scans over the fiber cell wall, showing secondary cell walls (SCW) and compound middle lamella (CML). Scale bars correspond to 10 µm.