Skip to main content
. 1999 Feb;65(2):540–548. doi: 10.1128/aem.65.2.540-548.1999

FIG. 1.

FIG. 1

FIG. 1

FIG. 1

Electron ionization mass spectra of the TMS derivatives of standard and E. coli-derived 3-hydroxyalkanoate methylesters. (A and B) TMS methyl esters of 3-hydroxyhexanoate (Mw 218) derived from a PHA standard from P. putida KT2442 (A) and from E. coli JMU193 (pET702, pCK01-alkS, pCY323) (B) grown on gluconate. Characteristic peaks: basis peak: m/e 203, M−15; m/e 145, M−73, m/e 133, (C5H13SiO2)+, m/e 131, (C5H11SiO2)+, m/e 89, (CH3)3SiO+, m/e 73, (CH3)3Si+. (C and D) TMS methyl esters of 3-hydroxyoctanoate (Mw 246) derived from a PHA standard from P. putida KT2442 (C) and from E. coli JMU193 (pET702, pCK01-alkS, pCY323) (D) grown on gluconate. Characteristic peaks: basis peak: m/e 231, M−15; m/e 175, (CH3)3SiO+⩵CHCH2CO2CH3; m/e 133, (C5H13SiO2)+; m/e 131, (C5H11SiO2)+; m/e 89, (CH3)3SiO+; m/e 73 (CH3)3Si+. (E and F) TMS methyl esters of 3-hydroxydecanoate (Mw 274) derived from a PHA standard from P. putida KT2442 (E) and from E. coli JMU193 (pET702, pCK01-alkS, pCY323) (F) grown on gluconate. Characteristic peaks: m/e 259, M−15; m/e 175 (CH3)3SiO+⩵CHCH2CO2CH3; m/e 133, (C5H13SiO2)+; m/e 131, (C5H11SiO2)+; m/e 89, (CH3)3SiO+; m/e 73, (CH3)3Si+.