FIG. 3.

Correlation of PHA accumulation and ′thioesterase I activity. E. coli JMU193(pET702, pCK01-alkS, pCY323) was cultivated in E2 minimal medium with 1% (wt/vol) gluconate, antibiotics, 0.01% (wt/vol) DCPK, and arabinose as indicated. The cells were harvested 25 h after reaching the stationary phase and lyophilized, and the PHA content was determined by GC (open circles). ′Thioesterase I activity (solid squares) was determined in crude extracts by spectrophotometric measurements and corrected by subtracting the ′thioesterase activity of crude extracts of E. coli JMU193 lacking the ′thioesterase I-encoding gene, from crude extracts of E. coli JMU193 expressing the ′thioesterase I-encoding gene.