TABLE 3.
PHA content and monomer composition of E. coli JMU193(pET702, pCK01-alkS pCY323)
| Inducer and concna | Amt of PHA (%, wt/wt) | PHA monomer compositionb (mol%)
|
||
|---|---|---|---|---|
| C6 | C8 | C10 | ||
| DCPK | ||||
| 0 | <0.1 | —c | — | — |
| 0.0025 | 1.2 ± 0.02 | 29 | 65 | 6 |
| 0.005 | 2.0 ± 0.01 | 27 | 67 | 6 |
| 0.02 | 1.9 ± 0.04 | 30 | 65 | 5 |
| 0.05 | 1.1 ± 0.01 | 33 | 62 | 5 |
| Arabinose | ||||
| 0 | <0.1 | — | — | — |
| 0.001 | 0.26 ± 0.006 | 33 | 64 | 3 |
| 0.01 | 1.24 ± 0.04 | 23 | 69 | 8 |
| 0.1 | 0.19 ± 0.06 | 21 | 71 | 8 |
| 2 | <0.1 | — | — | — |
a
Concentrations are percentages (vol/vol) for DCPK and percentages (wt/vol) for arabinose.
b
Cells were cultivated in E2 minimal medium with 1% gluconate, antibiotics, DCPK, and arabinose at the concentrations indicated. When varying the arabinose concentration, 0.01% DCPK was added, and when varying the DCPK concentration, 0.01% arabinose was added. Cells were harvested 25 h (arabinose variation) or 46 h (DCKP variation) after reaching the stationary phase and lyophilized, and the PHA composition was measured by GC. C6, 3-hydroxyhexanoate; C8, 3-hydroxyoctanoate; C10, 3-hydroxydecanoate.
c
—, not analyzed due to the low overall PHA content (<0.1%, wt/wt).