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Acetylsalicylic acid effect is dose-dependent. IF-GLA-L300F was treated with different doses of acetylsalicylic acid (range 0–3.2 mM) in the presence of 10 μM DGJ. AGAL specific activity measured on protein extracts is shown. Tukey’s HSD was used to evaluate significant differences among treatments (***: p < 0.001; **: p < 0.01; n = 2). AGAL activity increase upon combined treatment is dose-dependent. Immunoblots confirmed the results (D = DGJ 10 μM; D + A = DGJ 10 μM + ASA).
