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. 2022 May 4;23(9):5105. doi: 10.3390/ijms23095105

Figure 7.

Figure 7

Acetylsalicylic acid prolongs the effects of DGJ. IF-GLA-L300F cells were treated with different combinations of 10 μM DGJ and 4 mM acetylsalicylic acid (ASA), and the AGAL content was evaluated for seven days. (A) compares DGJ monotherapy versus the combined therapy with ASA; drugs were renewed every other day. In (B), the effect of ASA renewal every other day was compared to the single-dose treatment. AGAL specific activity measured on protein extracts is reported. Tukey’s HSD was used to evaluate significant differences among treatments only between treatments within the same day (***: p < 0.001; **: p < 0.01; n = 3). No comparison between treatments belonging to different days was made. Immunoblot analysis confirmed the results (U = untreated; D + A w ren = DGJ 10 μM + ASA 4 mM with ASA renewal; D + A w/o ren = DGJ 10 μM + ASA 4 mM without ASA renewal). Each panel includes the experimental design, specific activity, and an immunoblot for each experiment.