Figure 10.

Salicylic acid acts as acetylsalicylic acid. IF-GLA-MUTs, specifically D244H (A), L300F (B), V269M (C), Q280K (D, were treated for 72 h with the following drugs: i. untreated; ii. 10 μM DGJ; iii. 4 mM salicylic acid (sal); iv. 10 μM DGJ + 4 mM sal. AGAL specific activity measured on protein extracts is shown. Tukey’s HSD was used to evaluate significative differences among treatments (***: p < 0.001; **: p < 0.01; *: p < 0.05; n = 2). The effects of combined treatment (DGJ + sal) are significantly higher than those of DGJ monotherapy. Immunoblots confirmed the results (U = untreated; D = DGJ 10 μM; S = sal 4 mM; D + S = DGJ 10 μM + sal 4 mM). Each panel includes specific activity and an immunoblot for a cell line.