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. 2022 May 13;17(5):e0261743. doi: 10.1371/journal.pone.0261743

Fig 3. Selective expression of FN1 and COL6A4 in colonic MuPαC.

Fig 3

A and B: Restricted expression of FN1 and COL6A protein within colonic subepithelial PDGFRA+ cells. Anti-FN1 and anti-COL6A antibodies were used. Vertical and horizontal cross-sections (CS) images are indicated. Scale bars are 50 μm. C: Expression of Adamdec1, Fn1, and collagen type 6 isoforms (Col6a1-5) in Mu, isolated MuPαC, SM, and isolated SMPαC examined by RT-PCR. D: Quantitative analysis of Adamdec1, Fn1, and Col6a1-5 mRNA expression in Mu (n = 5), isolated MuPαC (n = 5), SM (n = 5), and isolated SMPαC (n = 6) measured by qPCR. ** p ≤ 0.01, MuPαC versus SMPαC. Gapdh was used as an endogenous control.