Figure 3. Disrupted enterocyte architecture in UNC45A deficiency.

(A) Confocal images of polarized NT control and Unc45A^KO Caco-2 cells grown on a filter and stained for an apical brush border marker DPPIV and actin. (B) Actin staining in polarized Caco-2 cells. Nuclei were visualized with HOECHST. Arrows on the left mark the corresponding XY and XZ planes. Scale bars: 20 μm. Panels A and B were from the same experiment. (C) NT control and UNC45A^KO Caco-2 cells complemented or not with WT or mutant alleles were cultured in 3D for 5 days to form cysts. Nuclei are stained with Nucblue (blue); actin is stained with phalloidin AF 455 (red). Single confocal sections through the middle of the cyst are shown. Scale bars: 10 μm. (D) Single-lumen cysts were counted in each experiment. Results from 3 independent experiments (35 cysts each) are shown, 1-way ANOVA. ****P < 0.0001.