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. 2022 May 16;132(10):e145889. doi: 10.1172/JCI145889

Figure 10. Kisspeptin inhibits triglyceride accumulation in isolated primary mouse hepatocytes.

Figure 10

(A) Representative confocal image of endogenous KISS1 immunostaining in control (CTRL) hepatocytes. Scale bars: 50 μm. (B and C) Effect of KP10 (100 nM) or KPA (3 nM) on triglyceride (TG) accumulation (expressed as fold change over vehicle) in (B) CTRL and (C) hepatic Kiss1r-knockout (LKO) hepatocytes treated with oleic and palmitic acid (150 μM each). (D) Expression of indicated genes by RT-qPCR. (E) Representative Western blots of indicated proteins in hepatocytes following KP10 (100 nM) or KPA (3 nM) treatment. Quantification of blots is shown in Supplemental Figure 13, A and B. (F and G) Representative Western blots of indicated proteins in hepatocytes in the presence or absence of (F) compound C (CC; 10 μM) treatment, with quantification of blots, as well as in the presence or absence of (G) YM-25489 (YM; 3 μM), with quantification of blots. *P < 0.05 versus controls; 1-way ANOVA followed by Dunnett’s post hoc test.