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. 2022 May 16;132(10):e145889. doi: 10.1172/JCI145889

Figure 9. KISS1R agonist alleviates NASH in DIAMOND mice fed WDSW.

Figure 9

Diet-induced animal model of nonalcoholic liver disease (DIAMOND) mice on Western diet with sugar water (WDSW) for 33 weeks were treated with vehicle (VEH; PBS) or kisspeptin agonist (KPA) for 6 weeks. (A) Liver triglycerides (TGs). (B) Representative liver histology for H&E and Oil Red O staining. Quantification of staining is shown. Scale bars: 500 μm. (C) Serum TGs levels. (D) Free fatty acid (FFA) levels and (E) cholesterol levels. (F) Expression of indicated genes by RT-qPCR. (G and H) Representative Western blot showing expression of indicated proteins. (I) β-Hydroxybutyrate levels. (J) Expression of indicated hepatic genes by RT-qPCR. Representative Western blot showing expression of indicated nuclear proteins in K. (L) DIAMOND mouse livers depleted of AMPK and (M) hepatic Kiss1r-knockout (LKO) HFD livers. Densitometric analyses of blots shown in Supplemental Figure 10, N–P. Data are shown as the mean ± SEM. Student’s unpaired t test; *P < 0.05 versus respective controls.