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. 2022 May 16;132(10):e147822. doi: 10.1172/JCI147822

Figure 5. H. pylori significantly alters bile acid levels under conditions of iron deficiency in INS-GAS mice.

Figure 5

Male INS-GAS mice were maintained on an iron-replete or iron-depleted diet and then challenged with Brucella broth or the wild-type H. pylori strain PMSS1. Mice were euthanized 8 weeks after challenge. Gastric tissue was processed for targeted bile acid analyses. Total bile acids (A), muricholic acids (B–F), cholic acids (G and H), and DCAs (I–N) were significantly increased by H. pylori under conditions of iron deficiency compared with infected mice under iron-replete conditions. n = 10 mice analyzed per group from 2 independent experiments. Median values are shown in box-and-whisker plots, with whiskers designating minimum and maximum values. A 1-way ordinary ANOVA with Šidák’s multiple-comparison test was used to determine statistical significance. Only statistically significant comparisons are denoted. *P < 0.05, **P < 0.01, and ***P < 0.001.