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. 2022 May 4;49(6):84. doi: 10.3892/ijmm.2022.5140

Figure 1.

Figure 1

Starved pancreatic cancer cells react differently to ferroptosis induction. (A-D) Viability assessment of (A) Miapaca2, (B) Panc-1, (C) Su.86.86 and (D) T3M4 cells cultured without FBS, without the amino acids L-glutamine, L-lysine and L-arginine or pseudo-starved using treatment with the mTOR inhibitors rapamycin (0.25 nM) and INK (0.125 nM). (E-H) Measurement of lipid peroxidation of (E) Miapaca2, (F) Panc-1, (G) Su.86.86 and (H) T3M4 cells cultured without FBS, L-glutamine, L-lysine and L-arginine and pseudo-starved using treatment with rapamycin (0.25 nM). Ferroptosis was induced by erastin and inhibited by Ferr-1. The data are presented as the mean ± SD; n=3. Cells cultured under standard conditions were used as the control. *P<0.05, **P<0.01 and ***P<0.001, vs. control. -AA, cells, cultured without L-glutamine, L-lysine and L-arginine; Rap, rapamycin; INK, INK128; Ferr-1, ferrostatin-1.