Figure 2.

ERK1/2 is translocated to the nucleus in starved pancreatic cancer cells. (A) Representative fluorescence microscopy images of (pseudo)starved (rapamycin-treated) Panc-1 cells, with or without erastin treatment. Magnification, ×20. (B) Quantification of the fluorescence intensity in cell nucleus of (pseudo)starved (Rap-treated) Miapaca2, Panc-1, Su.86.86 and T3M4 cells. The data are presented as the mean ± SD; n=3. Cells cultured under standard conditions were used as the control. ^*P<0.05 and ^**P<0.01 vs. control. -AA, cells, cultured without L-glutamine, L-lysine and L-arginine; Rap, rapamycin.