Figure 5.

Pancreatic cancer cells transition between different mesenchymal states during FBS starvation. (A) Representative images of Miapaca2 cells, starved for 2 and 5 days without FBS. Scale bar, 50 µm. (B) Wound healing assay of non-starved and 4 days starved Miapaca-2 cells. Scale bar, 200 µm. (C) Quantification of the results of wound healing assay in panel B. (D) Western blot analysis of vimentin in Miapaca2 cells, starved for 1-7 days. (E) Quantification of western blots in panel D. (F) Representative images of Capan-26 cells, starved for 3 days without FBS. Scale bar, 50 µm. (G) E-cadherin immunofluorescence of starved and non-starved Capan-26 cells. Scale bar, 50 µm. The data are presented as the mean ± SD; n=3. Cells cultured under standard conditions were used as the control. ^*P<0.05, ^**P<0.01 and ^***P<0.001, vs. 0 days of starvation.