TABLE 2.

Generation of free amino acids and natural dipeptides in sarcoplasmic protein extracts

Substrate	Amt of amino acid or dipeptide generated (mg/100 ml of extract)a
	Control	L. curvatus			L. sake
		Whole cells	CE	Cells + CE	Whole cells	CE	Cells + CE
Aspartic acid	6.41	0.02	−0.05	9.30	−0.29	−0.27	0.52
Glutamic acid	−22.89	−6.79	60.40	−8.22	−9.91	13.28	11.29
Serine	2.26	−2.75	3.74	−1.22	−2.77	0.61	1.62
Asparagine	0.38	−0.79	−1.14	0.73	−1.14	−0.90	−0.58
Glycine	2.75	−6.68	4.13	−5.31	−6.72	1.85	4.48
Glutamine	−17.97	−56.61	−99.87	−24.96	−55.67	−23.06	−18.66
β-Alanine	−0.04	−1.91	7.38	6.48	−1.86	12.05	15.30
Histidine	2.34	−2.63	17.64	−2.64	−2.39	2.67	0.44
GABAb	1.06	−0.63	0.77	27.19	−0.62	13.99	13.44
Threonine	5.39	25.72	−1.96	29.69	10.08	13.12	9.21
Alanine	7.07	−14.05	37.54	−13.16	−12.42	15.06	40.67
Arginine	0.57	−3.72	2.88	−1.10	−4.05	−1.76	−1.73
Proline	2.93	−4.66	1.12	−4.83	−4.63	2.55	4.92
Tyrosine	0.81	−1.67	1.25	7.76	0.65	1.32	1.40
Valine	2.11	−2.51	−1.88	−1.13	−2.38	3.01	3.87
Methionine	1.08	−1.53	1.79	0.27	−1.22	1.36	2.42
Isoleucine	1.13	−1.38	0.39	0.01	−1.28	2.01	2.36
Leucine	2.83	−2.62	2.53	0.27	−2.51	5.69	8.52
Phenylalanine	1.46	−1.85	2.37	7.90	−1.75	3.33	4.47
Tryptophane	0.27	−0.66	0.00	0.24	−0.66	0.86	1.21
Ornithine	0.63	−0.41	0.00	0.72	−0.55	2.87	5.53
Lysine	2.90	−3.49	7.72	1.74	−3.75	2.41	2.42
Anserine	0.31	−3.89	0.84	−12.02	−9.28	−0.51	1.19
Carnosine	−11.62	−118.47	−18.29	−244.36	−95.57	−8.92	−1.60
Taurine	0.52	−24.56	0.25	−14.80	−25.26	−9.51	−8.41

^aSarcoplasmic protein extracts were incubated at 37°C with whole cells, CE, and a combination of whole cells and CE. 

^bGABA, γ-amino butyric acid.