Figure 5.
CircPTP4A2 is targeted by miR-330-5p in HuMSC cells. (a) The levels of miRNAs (miR-487a, miR-330-5p, miR-1290, miR-326, miR421, miR-502-5p, miR-532-3p, miR-335, and miR-1305) in HuMSCs with or without circPTP4A2 knockout were analyzed using real-time PCR. (b) Ago2 coimmunoprecipitation assay and (c) miR-330-5p RNA pull-down were used to evaluate the interaction between emiR-330-5p and circPTP4A2. (d) Dual-luciferase reporter assay was used to evaluate the effect of miR-30-5p on circPTP4A2 in HuMSCs. N = 3. ∗P < 0.05 relative to the indicated group.