Table 12.

Cytotoxicity and mutagenicity of LN18178 in mouse lymphoma cells, L5178Y TK^+/−

Experiment duration	Concentration (μg/mL)	Metabolic activation system	RSG (%)	RCE (%)	RTG (%)	Cloning efficiency (CEV)	Cloning efficiency (CEM)	Mutant frequency (10−6 cells)	% SC
3 hr	0 (NC)	−	118.20	90.56	107.05	1.00	72.79	73	24
		+	116.22	87.16	101.30	0.97	111.96	118	16
	0 (VC)	−	100.00	100.00	100.00	1.10	95.89	87	17
		+	100.00	100.00	100.00	1.11	108.72	98	15
	62.5	−	80.61	96.73	77.97	1.06	118.59	111	20
		+	79.19	93.96	74.41	1.05	94.38	90	20
	125	−	71.65	96.52	69.16	1.06	97.45	92	26
		+	56.50	88.04	49.74	0.98	102.53	104	21
	250	−	39.71	93.25	37.03	1.03	104.08	105	12
		+	29.59	86.16	25.50	0.96	123.44	129	23
	500	−	19.16	91.98	17.62	1.01	125.91	125	11
		+	12.55	100.82	12.66	1.12	77.41	71	19
	0.1 (NQO)	−	50.17	77.54	38.90	0.85	402.42	472	57
	5 (CPC)	+	30.37	81.56	24.77	0.91	500.45	569	49
24 hr	0 (NC)		115.91	87.34	101.23	0.90	75.78	86	9
	0 (VC)		100.00	100.00	100.00	1.03	102.43	99	21
	15.63		68.75	106.14	72.97	1.10	83.52	76	15
	31.25	−	47.31	102.94	48.70	1.06	139.32	131	20
	62.50		31.99	100.13	32.03	1.03	126.82	123	14
	125.00		15.58	98.49	15.34	1.02	99.06	98	22
	0.1 (NQO)		31.77	79.58	25.28	0.81	681.69	831	65

^∗ NC, negative control (water); VC, vehicle control (1%, DMSO); NQO, 4-nitroquinoline-1-oxide; CPC, cyclophosphamide; SC, small colony; RSG, relative suspension growth; RCE, relative cloning efficiency; RTG, relative total growth; CE_V, cloning efficiency (viable count) plates, CE_M, cloning efficiency mutant selection plates.