Extended Data Fig. 7. SOX2 promotes nucleosome eviction from peaks containing high scoring motifs.

(a) Metaplots of ATAC-seq data at cell-type specific SOX2 bound CREs classified in Fig. 3e. Naïve ATAC-seq data reanalysed from⁶³. Metaplots show mean ± s.e.m. (b) SOX2 occupancy in CEpiLCs and EpiLCs at cell-type specific CREs classified in Fig. 3e. (c) Percentage of peaks from each cluster with at least 1 SOX2 motif with the indicated number of mismatches. (d) Average number of motifs per peak in each cluster with less than or equal to the indicated number of mismatches. (e) Nucleosome occupancy and SOX2 ChIP occupancy profiles of individual cluster 3 peaks ranked by their total FIMO motif score. Higher scoring peaks show a higher intensiy of SOX2 ChIP signal and a greater degree of nucleosome depletion at SOX2 peak centres in SOX2-ON.