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. 2022 May 12;24(5):633–644. doi: 10.1038/s41556-022-00910-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Epiblast-like cells differentiated in FL medium recapitulate in vivo gene-expression dynamics⁶⁵. Illustrative genes for each cluster are highlighted. Gene expression profiles from 3 biological replicates are shown. See methods for details of genes plotted. (b) Flow cytometry analysis shows that culture in FLC medium reduces SOX2 levels and induces T/BRA. (c) Measurement of relative mRNA expression by RT-qPCR shows that FLC medium induces caudal epiblast markers Cdx1,2,4, and (d) posterior Hox genes. Normalised mean expression is shown relative to peak expression. Line is a loess fit to the data. The number of biological replicates averaged to fit each point are shown in Source numerical data available in source data.

Source data