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Western blot analysis of PepI. Immunoblot analyses of wild-type PepI (lanes 3 and 4), PepI 1-65 (lanes 5 and 6), F13D-I17R PepI (lanes 7 and 8), and I17R PepI (lanes 9 and 10) in soluble and membrane cytoplasmic fractions of cell extracts, respectively, are shown. Standard peptides (sizes are indicated to the left of the gel in kilodaltons) and synthetic PepI are shown in lanes 1 and 2, respectively.
