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. 2022 May 13;13:2661. doi: 10.1038/s41467-022-30401-9

Fig. 3. Loss of salm initiates sarcomere branching through tubular conversion of indirect flight muscles.

Fig. 3

a 3D rendering of myofibrillar matrix in IF muscles with (b) and without (a) Mef2-Gal4 driven RNAi knockdown of salm. Bottom two images show clippings through the long axis of the muscle directly above. c Assessment of myofibril size and shape for IF and Salm KD IF muscles. d Percentage of myofibrils with at least one branching sarcomere (left) and percentage of sarcomeres per myofibril with a branch (right). e Frequency of sarcomere branching in IF and Salm KD IF muscles. N values:: IF—3 muscle cells, 3 datasets, 114 myofibrils, 982 sarcomeres; Salm KD IF—3 muscle cells, 3 datasets, 180 myofibrils, 932 sarcomeres. Larger shape symbols represent data from a single cell and smaller shape symbols represent data from a single myofibril. Bars represent muscle cell overall mean ± SE. Hash sign (#): Significantly different from fibrillar IF (two-sided, independent t-test, P < 0.05). Scale bars—1 μm.