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. 2022 May 13;13:2661. doi: 10.1038/s41467-022-30401-9

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2022

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Fig. 5 — a–e Confocal microscopic images of adult indirect flight muscle (IFM) actin after different developmentally timed knockdowns (KD) of neurochondrin (NCDN). Blue dotted lines highlight the region shown in inset (lower panels). f–u Confocal microscopy images of IFM actin during pupal development in wild type and NCDN KD flies. Cross-section images are shown at the left of each image and dotted lines highlight the zoomed-in regions shown to the right of each image. Representative of cells from three flies. Scale bars: 5 µm. v Flight test results in wild type (WT) and different developmentally timed NCDN KD flies. w IFM actin (Act88F) and myosin (Mhc) relative RNA expression levels in adult WT and Mef2-Gal4::UAS-NCDN^RNAi flies. x IFM Act88F and Mhc relative RNA expression levels in adult WT, Act88F-Gal4::UAS-NCDN^RNAi, and Mhc-Gal4::UAS-NCDN^RNAi flies. Bars represent overall mean ± SE from three samples (individual points) of 20 pooled thoraces per group. Asterisks (*): Significantly different between groups (w two-sided, independent t test, x one-way ANOVA with Tukey’s HSD post hoc test, P < 0.05).