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. 2022 May 13;8:41. doi: 10.1038/s41522-022-00301-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Community structure assessed via 2nd order Hill α-diversity (²D, upper panels) and community assembly evaluated via the nearest taxon index (NTI, lower panels), from bacterial ASV data for different frequencies of organic loading disturbance (n = 5). Disturbance frequency levels (L): 0 (undisturbed), 1–4 (intermediately disturbed), 5 (press disturbed). In: sludge inoculum (day 0, n = 4). Each panel represents a sampling day, red diamonds display mean values. The box bounds the interquartile range (IQR) divided by the median, and Tukey-style whiskers extend to a maximum of 1.5 times the IQR beyond the box. Characters above boxes display Games-Howell post hoc grouping (P_adj < 0.05). Welch’s ANOVA P-values adjusted at 5% FDR shown within panels. Correlations of B ²D and C phylogenetic diversity (PD) vs. NTI from bacterial ASV data across all frequency levels and time points evaluated in this study (m = 184). Kendall correlation τ- and adjusted P-values are indicated within the panel. Blue line represents locally estimated scatterplot smoothing regression (loess) with confidence interval in dark-gray shading. Note the inverted y-axis for NTI, as values closer to zero indicate a higher relative contribution of stochastic assembly. Shaded in gray is the zone of significant stochastic phylogenetic dispersion, |NTI | < 2.