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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Br J Pharmacol. 2022 Feb 7;179(12):2953–2968. doi: 10.1111/bph.15779

FIGURE 2.

FIGURE 2.

Effect of potassium channel inhibitors on Ieq in mCCDcl1 cells. (a) Effect of basolateral application of 100 μM fluoxetine (n = 8) and 50 μM amitriptyline (n = 7) on Ieq measured at different time points. One Way RM ANOVA with a Greenhouse-Geisser correction (F(1.81, 10.87) = 14.03, P < 0.002), post hoc test using Dunnett correction *P < 0.05 compared to starting point. (b) Effect of apical or basolateral application of 50 μM amitriptyline on amiloride-sensitive Na+ epithelial transport in mCCDcl1 cells (n = 5 for each group, One Way ANOVA, Dunnett post-hoc test *P < 0.05 compared to control). (c) Concentration-dependence of amitriptyline effect on Ieq (n = 6 per point). (d) Modulation of Ieq by different concentrations of VU992 (vehicle, n = 11; 3 μM, n = 7; 30 μM, n = 8; 300 μM, n = 8). (e) Summary graph for the ENaC current modulation (Ieq) by Kir channels blockers (vehicle, n = 14; fluoxetine, n = 8; amitriptyline, n = 9; VU992, n = 17). Ieq for (d) and (e) recorded 2 hrs after drugs or vehicle application (One Way ANOVA, Dunnett post-hoc test, *P < 0.05 compared to vehicle).