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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Br J Pharmacol. 2022 Feb 7;179(12):2953–2968. doi: 10.1111/bph.15779

FIGURE 5.

FIGURE 5.

Effect of amitriptyline on Kir4.1/Kir5.1 and ENaC single-channel activity in principal cells of freshly isolated rat CCD. Representative patch-clamp configurations (glass pipette forming a giga-seal contact) and corresponding recordings of basolateral Kir4.1/Kir5.1 (a) and apical (split-open tubule) ENaC (b) single-channel activity recorded from the CCD principal cells after application of PSS as a vehicle control or application of amitriptyline. Scale bars are 100 μm. Patches were clamped to -Vp = −60 mV. Summary graphs of changes in channel’s activity for Kir4.1/Kir5.1 (n = 8) and ENaC (n = 9) channels are shown on the right. Paired sample t-test *P < 0.05.