Anti-mouse β–III tubulin (Tuj) antibody was used to label neurite projections in vertically sectioned mouse retinas. a) Representative images of Tuj staining in vertical sectioned retina from 8-week-old and 10-week old mice. b) Quantification of Tuj staining in GCL layer of a) (F=48.50, p<0.0001. For 8-week: Naïve vs BCRMOG, P=0.0909; Naïve vs OSE, P<0.0001; BCRMOG vs OSE, P<0.0001. For 10-week: Naïve vs BCRMOG, P=0.6265; Naïve vs OSE, P<0.0001; BCRMOG vs OSE, P<0.0001). c) Quantification of Tuj staining in IPL layer of a) (F=38.78, p<0.0001. For 8-week: Naïve vs BCRMOG, P=0.0807; Naïve vs OSE, P<0.0001; BCRMOG vs OSE, P<0.0001. For 10-week: Naïve vs BCRMOG, P=0.0497; Naïve vs OSE, P<0.0001; BCRMOG vs OSE, P<0.0001). Each dot from bar graph represents one mouse. Significance of Tuj intensity level between groups was assessed by one-way ANOVA (* P ≤ 0.05, *** P ≤ 0.001, N.S. = no significance). Error bars represent SEM. Scale bar =50μm.