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. Author manuscript; available in PMC: 2022 Oct 29.
Published in final edited form as: Nat Immunol. 2022 Apr 29;23(5):705–717. doi: 10.1038/s41590-022-01192-4

Extended Data Fig. 7. dTGN colocalization and biochemical procaspase-11-NLRP3 interaction.

Extended Data Fig. 7

a,b, Immunofluorescence confocal microscopy 16hr post-stimulation of WT macrophages with L or HK virulent E. coli (a), and WT, Casp11–/– and Nlrp3–/– macrophages as indicated with L E coli (b). Scale bar=10μm. In (a), side micrograph insets in the triple merges show magnification of the indicated areas. c,d, Immunoprecipitation (IP) of endogenous caspase-11 or NLRP3 as indicated, and immunoblotting for co-immunoprecipitating proteins and WCE proteins (labels to left of immunoblot panels) from macrophages stimulated 12hr with L, HK or HK E. coli supplemented with indicated dose of E. coli RNAtot. (c) and L, HK or HK E. coli supplemented with indicated dose of E. coli RNAtot or Nigericin, E. coli RNAtot alone or Nigericin alone (d). Bacteria:macrophage=20:1. Results represent at least 3 independent experiments.