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PAGE of rSapSh. Lane 1, precipitate obtained from cell extract of E. coli BL21(DE3)/pSapSh3; lane 2, soluble active preparation of rSapSh after bacitracin-Sepharose column chromatography; lane 3, molecular weight markers. The position of the inclusion body formed from the preproprotein of rSapSh is indicated by an arrow.
