TABLE 2.

Properties of SapSh and subtilisin Carlsberg determined from a comparison of the deduced amino acid sequences

Enzyme	Amino acid frequencies (%)a						pI	GRAVY indexb	Aliphatic index
	Charged amino acids	Acidic amino acids	Basic amino acids	Polar amino acids	Hydrophobic amino acids	Aromatic amino acids
Subtilisin Carlsberg	11.7	5.1	4.7	28.1	37.2	6.6	6.57	0.132	84.78
SapSh	13.4	7.0	4.2	34.0	32.4	6.3	5.07	−0.157	75.38

^aThe charged amino acids are arginine, lysine, histidine, aspartic acid, and glutamic acid; the acidic amino acids are aspartic acid and glutamic acid; the basic amino acids are lysine and arginine; the polar amino acids are asparagine, cysteine, glutamine, serine, and threonine; the hydrophobic amino acids are alanine, isoleucine, leucine, phenylalanine, tryptophan, and valine; and the aromatic amino acids are phenylalanine, tryptophan, and tyrosine. 

^bGRAVY, Grand Average of Hydropathicity.