Figure 3.

ACE2‐containing EVs inhibit pseudotyped SARS‐CoV‐2 transduction. A) Cartoon depicting pseudotype lentivirus assay. Transduction of ACE2⁺ HEK293FT cells by SARS‐CoV‐2 Spike pseudotyped virus results in expression of EYFP as a reporter of viral entry. B) Representative relative frequency histograms of EYFP expression of ACE2⁺ or parental HEK293FT cells after exposure to no virus, mock virus (no surface glycoprotein), or Spike‐pseudotyped virus. C) Cartoon depicting pseudotype lentivirus inhibition assay. Effective inhibitors of viral transduction reduce the number of cells expressing EYFP. D) Dose‐response curve demonstrating the relationship between EV dose and normalized percentage of cells transduced by Spike‐lenti. Curves are normalized to the percent of cells transduced at the lowest EV dose in a particular curve. Symbols represent the mean of three biological replicates except for the ACE2 negative, HS‐EV condition, where the third and fourth dilutions in series (2.7 × 10⁷ particles and 6.6 × 10⁶ particles, respectively) are the mean of two biological replicates. Error bars are standard error of the mean. Data are representative of two independent experiments.