Figure 5.

Decoy vesicles are robust to drug‐resistant Spike mutations. A) Cartoon depicting the SARS‐CoV‐2 Spike variants investigated in this study and their relative susceptibility to inhibition by soluble ACE2 or monoclonal antibody treatments. B) Top: Dose‐response curves depicting the inhibition of various strains of SARS‐CoV‐2 Spike pseudotyped lentivirus against soluble ACE2, WT‐ACE2 UC‐EVs, and Mut‐ACE2 UC‐EVs. Curves are normalized to the percent of cells transduced at the lowest EV dose in a particular curve. Symbols represent the mean of three biological replicates; error bars are standard error of the mean. Data are representative of two independent experiments. B) Bottom: Log(ID₅₀) values calculated from the data in the top portion of this panel. Numbers above each point report the relative resistance for a given strain relative to the parental (D614G) strain for that particular inhibitor treatment. Error bars represent 95% confidence intervals for the parameter (ID₅₀) estimation.