A: Timeline of experimental procedures for Experiment 1a. Rats first received two sessions of auditory fear conditioning in Context A, followed by a single exposure session to Context A. Then, rats underwent 6 sessions of either conditioned stimulus (tone) extinction in Context B, followed by an additional exposure session to Context A. Rats were then tested for fear to the tone in Context A and Context B. Grey shaded region represents CNO injections prior to behavioral testing. B: Post-shock freezing during the two conditioning sessions, followed by freezing during the first session of Context A exposure. Freezing to the tone during the 6 extinction sessions, followed by freezing during the second Context A exposure session. C: freezing to the tone in both Context B and Context A. CNO was injected 30 minutes prior to these sessions. “*” = significant difference (p < .05) between GFP and hM4Di in Context A. “#” = significant main effect of group collapsed across contexts (p < .05).