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. Author manuscript; available in PMC: 2022 May 14.
Published in final edited form as: Nat Med. 2022 Feb 21;28(4):766–779. doi: 10.1038/s41591-022-01680-y

Fig. 6 |. An increase in gut-homing PBs/PCs is associated with disease complications and activity in two validation cohorts of patients with active UC.

Fig. 6 |

a–d, Mass cytometry (CyTOF)-derived data from VCo1 comparing PC parameters among HCs (n = 65), patients with quiescent UC (n = 52) and patients with inflamed UC (n = 31). a, Representative tSNE plots (top) demonstrating cellular subsets in the peripheral blood of one HC, one patient with quiescent UC and one patient with inflamed UC. The bottom panels compare the expression of β7-integrin on CD27+CD38hi PBs/PCs. b, Cumulative data from VCo1 comparing the frequency of PBs/PCs among HCs and patients with quiescent UC and patients with inflamed UC; Mann–Whitney test, two-tailed. c, Mean level of expression of β7-integrin (left), β1-integrin (middle) and CXCR3 (right) on circulating PBs/PCs of HCs and patients with quiescent UC and patients with inflamed UC. Data are shown as individual data, mean and standard deviation; Mann–Whitney test, two-tailed. d, Kaplan–Meier estimates showing survival free of occurrence of complications (that is, intestinal surgery, UC-related hospitalization or introduction of a new biologic treatment) for patients of VCo1 with PB/PC frequency below the median (red curve) or above the median (blue curve). Survival curves were compared using a log-rank test, n = 73 patients with UC. e–g, IgJ gene signatures, representing circulating PBs/PCs in VCo2 comprised of 36 HCs and 107 patients with UC. e, Relative expression of IgJ gene from whole blood of HCs, patients with quiescent UC and patients with inflamed UC. f, g, Relative expression of IgJ compared with rectal bleeding scores (f) and fecal lactoferrin (g). In b, e and f, box plots represent the third quartile (top of the box), median (center lines) and first quartile (bottom of the box) of measurements, and the whiskers represent 1.5 times the interquartile range from the top or bottom of the box. Data are shown as individual data. Comparisons between indicated groups were made with two-tailed t-test. Correlation coefficient (rho) in g was calculated using Spearman’s test. P values are as indicated throughout the figure. tSNE, t-distributed stochastic neighbor embedding.