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. 2022 May 14;18:178. doi: 10.1186/s12917-022-03256-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effect of dietary Nano curcumin and Saccharomyces cell wall on immunostaining of NFkB P65 of the liver for broiler chicks fed on diet containing 0.25 mg aflatoxin B1 / kg diet at 1 to 35 day where (A) G1: showing mild immunostaining of NFkB P65 antibody within the hepatocytes (arrowheads), (B) G2: showing marked cytoplasmic and nuclear expression of NFkB P65 antibody within the hepatocytes (arrowheads), NFkB P65 IHC, (C) G3: showing decrease the expression of NFkB P65 antibody within the hepatocytes (arrowheads), (D) G4: showing decrease the expression of NFkB P65 antibody within the hepatocytes (arrowheads), NFkB P65 IHC, (E) G5: Liver of of aflatoxin + combination group showing marked decrease the NFkB P65 expression within the hepatocytes (arrowheads), (F) showing percentage of NFkB P65 positive cells in different groups, NFkB P65 IHC, X200, bar = 40 µm. B: nfkb p65 labelling index