Fig. 6.

Targeting mtROS or SIRT1 regulated NOX4, SOD2, PPARα and CPT1a of LF in vivo. Mice exposed to CS were treated with MitoQ or SRT1720 at the start of smoking. 4 weeks later, lungs were harvested. A–C The expression of collagen I in lungs was examined by immunohistochemistry and CPT1a, PPARα, SIRT1, NOX4 and SOD2 level in LF (collagen I-positive cells) were detected by immunofluorescence. D CS decreased SIRT1 to activate LF by promoting mitochondrial oxidative stress, which dysregulated FAO and mitophagy through impairing autophagy flux. CS cigarette smoking