FIG. 1.

Effects of mutations on soluble rHb accumulation. Fermentations were incubated at 28°C, induced for 16 h with 100 μM IPTG, and supplemented with hemin (final concentration, 0.63 mM) at 0, 3, 6, 9, and 12 h after induction. (A) Soluble accumulation of four variants, rHb0.1 (SGE2706), rHb1.1 (SGE1464), rHb9+1.1 (SGE2782), and rHb9.1 (SGE2784), for 16 h after induction. Most symbols represent the average from three fermentations; the only exception is the SGE2782 symbols, which represent the average from five fermentations. (B) Accumulation of three variants of soluble rHb with a di-α K158C mutations, as described above. Most symbols represents the average from four fermentations; the only exception is the SGE3083 symbols, which represent the average from two fermentations.