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. 2022 May 14;12:8007. doi: 10.1038/s41598-022-12076-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphorylation levels of EGFR and IGF1R/INSR were higher in lenvatinib-resistant cells. (A–D) The Proteome Profiler Human Phospho-RTK Array Kit was used to determine the relative levels of tyrosine phosphorylation of human receptor tyrosine kinases (RTKs) in Hep3B cells (A), LR7.5-3B cells (B), Hep3B cells treated with 3 µM lenvatinib for 4 h (C), and LR7.5-3B cells treated with 3 µM lenvatinib for 4 h (D). (E) Immunoblotting analysis of phosphorylated EGFR (p-EGFR) and ACTB levels in Hep3B (3B) and LR7.5-3B (LR) cells that had been treated with or without 3 μM lenvatinib for 4 h. (F) Immunoblotting analysis of phosphorylated INSR (p-INSR) and ACTB levels in Hep3B (3B) and LR7.5-3B (LR) cells that had been treated with or without 3 μM lenvatinib for 4 h. The intensity with the internal control were indicated. Two independent experiments of (E,F) were performed, and representative results are shown.