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. 2022 May 14;12:8007. doi: 10.1038/s41598-022-12076-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the IGF1R inhibitor linsitinib on ERK phosphorylation and cell proliferation. (A) Immunoblotting analysis of phosphorylated ERK (p-ERK), ERK and ACTB levels in Hep3B and LR7.5- 3B cells that had been treated with linsitinib for 4 h. (B,C) Cell proliferation was analyzed by Cell Counting Kit 8 (CCK-8) assays in Hep3B (3B) (B) and LR7.5-3B (LR) (C) cells that had been treated with or without 0.1 μM linsitinib at the indicated times (n = 4 per treatment). Representative results are shown. (D) Immunoblotting analysis of phosphorylated ERK (p-ERK), ERK and ACTB levels in Hep3B and LR7.5-3B cells that had been treated with or without 3 μM lenvatinib, alone or combined with the indicated concentration of linsitinib for 4 h. (E,F) Cell proliferation was analyzed by Cell Counting Kit 8 (CCK-8) assay in Hep3B (3B) (E) and LR7.5-3B (LR) (F) cells treated with or without 3 μM lenvatinib, alone or combined with 0.1 µM linsitinib, at the indicated times (n = 4 per treatment). The intensity with the internal control were indicated. Two independent experiments were performed, and representative results are shown. *p < 0.05.